The style of the Madonna window, a late work of the Tiffany Studios and the last Tiffany window to be installed at Trinity, stands apart from the earlier windows. The glass is thinner, the palette has deepened to rich, dark colors and the drapery glass is not as textured.

Window installed in memory of Mary Caperton Braxton Holt (1863-1935) and bears a black, script-style shop signature "Louis Comfort Tiffany", 1937.